Products/Services Used | Details | Operation |
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Peptide Synthesis | ... recombinant C-terminal FGF23 fragment, Ser180-Val251 (N-terminal His-tagged, expressed in E. coli), was generated as a custom synthesis by Genscript (Piscataway, NJ, USA). We ... recombinant intact (#2629-FG) and C-terminal peptides (Genscript). Neither intact nor C- ... | Get A Quote |
In kidney disease, higher circulating levels of the mineral-regulating hormone fibroblast growth factor (FGF)-23 are predictive of disease progression but direct pathogenic effects on the kidney are unknown. We sought evidence of local renal synthesis in response to unilateral ureteric obstruction in the mouse, and pro-fibrotic actions of FGF23 on the fibroblast in vitro. Acute tubulointerstitial injury due to unilateral ureteric obstruction stimulated renal FGF23 synthesis by tubules, and downregulated inactivating proprotein convertases, without effects on systemic mineral metabolism. In vitro, FGF23 had divergent effects on fibroblast activation in cells derived from normal and obstructed kidneys. While FGF2... More