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Characterization of a putative ArsR transcriptional regulator encoded by Rv2642 from Mycobacterium tuberculosis.

J Biomol Struct Dyn.. 2017-07;

Q Li, C Li, L Xie, C Zhang, Y Feng, J Xie.

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Proteins, Expression, Isolation and Analysis	... were incubated for 4h at 37℃. Cell lysates were prepared by sonication, and His-Rv2642 was purified by binding to Ni-NTA (GenScript) equilibrated with wash Buffer (50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0), and eluted into ...	Get A Quote

摘要

178 HlyT from Vibrio cholerae is a positive regulator of Na(+)/H(+) antiporter, important for the survival of the organism in an aquatic environment and within the human host. Here we report cloning, over-expression and purification of HlyT.