Products/Services Used | Details | Operation |
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Codon Optimization | ... res.in/raghava/tappred). The amino acid sequence of the hCD20EXL reverse translated into nucleotide sequence, and codons were optimized by GenScript service (https://www.genscript. com/ssl-bin/quote). At the end, secondary ... | Get A Quote |
Design and production of monoclonal antibody for the diagnosis and immunotherapy of non-Hodgkin lymphoma require a suitable CD20 antigen as an effective immunogen. In this study, a new chimeric human CD20 extra loop (hCD20EXL) protein was designed by bioinformatics tools and was expressed in Escherichia coli BL21 DE3. Amino acid sequences, protein structure, immunogenicity, and other physicochemical property of potential antigens were in silico analyzed. Antigenicity, codon optimization, and other predictions of designed protein were determined by bioinformatics tools. The designed protein was heterologously expressed in E. coli and verified by SDS-PAGE and Western blot. Immunogenicity of this antigen was teste... More