| Products/Services Used | Details | Operation |
|---|---|---|
| Codon Optimization | ... no. AFD98306.1). Restriction sites for SacII and HindIII were added to the 5′ and 3′ ends, respectively. After codon optimization, the gene construct was synthesized (GenScript), ligated into the pHUE expression vector (Catanzariti et al. ... | Get A Quote |
A multiplex fluorescence microsphere immunoassay (FMIA) was used to detect bovine and ovine IgM and IgG antibodies to several Rift Valley fever virus (RVFV) proteins, including the major surface glycoprotein, Gn; the nonstructural proteins, NSs and NSm; and the nucleoprotein, N. Target antigens were assembled into a multiplex and tested in serum samples from infected wild-type RVFV or MP12, a modified live virus vaccine. As expected, the N protein was immunodominant and the best target for early detection of infection. Antibody activity against the other targets was also detected. The experimental results demonstrate the capabilities of FMIA for the detection of antibodies to RVFV structural and nonstructural p... More
