Products/Services Used | Details | Operation |
---|---|---|
Proteins, Expression, Isolation and Analysis | ... Next 100 μl of serially diluted recombinant CD19xCD3-bispecific engager protein (GenScript, Piscataway, NJ) or conditioned media (obtained from ENG T cells cultured for 24 hour at a concentration of 1 × 10 6 /mL) were added. ... | Get A Quote |
T cells expressing chimeric antigen receptors (CARs) or the infusion of bispecific T-cell engagers (BITEs) have shown antitumor activity in humans for CD19-positive malignancies. While BITEs redirect the large reservoir of resident T cells to tumors, CAR T cells rely on significant in vivo expansion to exert antitumor activity. We have shown that it is feasible to modify T cells to secrete solid tumor antigen-specific BITEs, enabling T cells to redirect resident T cells to tumor cells. To adapt this approach to CD19-positive malignancies we now generated T cells expressing secretable, CD19-specific BITEs (CD19-ENG T cells). CD19-ENG T cells recognized tumor cells in an antigen-dependent manner as judged by cyto... More