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Proteins, Expression, Isolation and Analysis | ... The viability of the chondrocytes in the alginate hydrogels after different compressive stimulation regimes was evaluated using Hoechst 33,342/Propidium Iodide (PI) Double Stain Apoptosis Detection Kit (Cat. L00309,GenScript,Piscatway,NJ,USA). ... | Get A Quote |
Biomechanics plays a critical role in the modulation of chondrocyte function. The mechanisms by which mechanical loading is transduced into intracellular signals that regulate chondrocyte gene expression remain largely unknown. Histone deacetylase 4 (HDAC4) is specifically expressed in chondrocytes. Mice lacking HDAC4 display chondrocyte hypertrophy, ectopic and premature ossification, and die early during the perinatal period. HDAC4 has a remarkable ability to translocate between the cell's cytoplasm and nucleus. It has been established that subcellular relocation of HDAC4 plays a critical role in chondrocyte differentiation and proliferation. However, it remains unclear whether subcellular relocation of HDAC4... More