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Proteins, Expression, Isolation and Analysis | ... Page 3. MOLECULAR MEDICINE REPORTS 3 Horseradish Peroxidase Substrate kit (GenScript, Piscataway, NJ, USA) and analysis was performed using Image J software v 1.49 (National Institute of Health, Bethesda, MD, USA). ... | Get A Quote |
Indoleamine 2,3‑dioxygenase (IDO), through L‑tryptophan depletion, activates general control non‑derepressible (GCN) 2 kinase and suppresses T‑cell proliferation, in addition to suppressing aerobic glycolysis and glutaminolysis, which are required for these rapidly proliferating cells. A number of, however not all of these alterations, are partially mediated through IDO‑induced p53 upregulation. In two‑way mixed lymphocyte reactions (MLRs), IDO reduced cellular proliferation. In MLR‑derived T‑cells, IDO induced the expression levels of p53 and p21, however concurrently reduced the levels of ζ‑chain, c‑Myc, lactate dehydrogenase A (LDH‑A) and glutaminase (GLS)2. However, p53 had no effect ... More