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Binding and entry of Clostridium difficile Toxin B is mediated by multiple domains.

FEBS Lett.. 2015-12;

Manse JS, Baldwin MR.

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Codon Optimization	... Inc. or Sigma-Aldrich. 2.1. TcdB protein expression and purification in Bacillus megaterium. Codon optimized DNA encoding full length TcdB (Gene ID: 4914074) was chemically synthesized by GenScript USA Inc. and ligated ...	Get A Quote

摘要

Clostridium difficile is responsible for a number of serious gastrointestinal diseases caused primarily by two exotoxins, TcdA and TcdB. These toxins enter host cells by binding unique receptors, at least partially via their combined repetitive oligopeptides (CROPs) domains. Our study investigated structural determinants necessary for binding and entry of TcdB. Deletion analyses identified TcdB residues 1372-1493 as essential for cytotoxicity in three cell lines. Consistent with this observation, overlapping TcdB fragments (residues 1372-1848, 1372-1493 and 1493-1848) were able to independently bind cells. Our data provide new evidence supporting a more complex model of clostridial glucosylating toxin uptake th... More