Products/Services Used | Details | Operation |
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Proteins, Expression, Isolation and Analysis | ... Yu and Dr. Hsu (Chang Gung University, Taiwan; Hsu et al., 2010) or from Genscript; NM2A (PRB-440P, Covance); paxillin (SAB4502553, Sigma); Myo1b (HPA013607, Sigma); phospho-myosin light chain (Cell Signal); Myo9b (Proteintech); and glyceraldehyde 3-phosphate ... | Get A Quote |
We investigated the myosin expression profile in prostate cancer cell lines and found that Myo1b, Myo9b, Myo10, and Myo18a were expressed at higher levels in cells with high metastatic potential. Moreover, Myo1b and Myo10 were expressed at higher levels in metastatic tumors. Using an siRNA-based approach, we found that knockdown of each myosin resulted in distinct phenotypes. Myo10 knockdown ablated filopodia and decreased 2D migration speed. Myo18a knockdown increased circumferential non-muscle myosin 2A-associated actin filament arrays in the lamella and reduced directional persistence of 2D migration. Myo9b knockdown increased stress fiber formation, decreased 2D migration speed, and increased directional pe... More