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Proteins, Expression, Isolation and Analysis | ... Japan) at 200 V for 1 hour. Coomassie Brilliant Blue staining of the gel was performed with e-Stain 2.0 Protein Staining System using eStain Protein Staining Pad (GenScript, NJ, USA). Detected bands were digitized by an image ... | Get A Quote |
Proteolytic stability in gastrointestinal tract and blood plasma is the major obstacle for oral peptide drug development. Inhibitor cystine knots (ICKs) are linear cystine knot peptides which have multifunctional properties and could become promising drug scaffolds. ProTx-I, ProTx-II, GTx1-15, and GsMTx-4 were spider-derived ICKs and incubated with pepsin, trypsin, chymotrypsin, and elastase in physiological conditions to find that all tested peptides were resistant to pepsin, and ProTx-II, GsMTx-4, and GTx1-15 showed resistance to all tested proteases. Also, no ProTx-II degradation was observed in rat blood plasma for 24 hours in vitro and ProTx-II concentration in circulation decreased to half in 40 min, in... More