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Proteins, Expression, Isolation and Analysis | ... MATERIALS AND METHODS. Reagents. Plasmid Miniprep Kit was purchased from BIOMIGA (San Diego, USA). Ni-NTA column was obtained from Genscript (NJ, USA). ... The protein was purified on a Ni-NTA column (Genscript, New Jersey, USA). ... | Get A Quote |
Streptococcus suis serotype 2 (SS2) is an emerging zoonosis, and meningitis is the most frequent clinical manifestation, but mechanism of its virulent factor, enolase (Eno), is unknown in meningitis. In this study, Eno was inducibly expressed and added to an in vitro Transwell co-culture model of the blood-brain barrier (BBB) consisted of porcine brain microvascular endothelial cells (PBMECs) and astrocytes (ACs), the results showed that Eno induces a significant increase in BBB permeability and promotes the release of IL-8 et al. cytokines. Furthermore, IL-8 could significantly destroy the integrity of the BBB model in vitro. In mice models administered Eno for 24 h, Eno could significantly promote Evans blue... More