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Development of a Cell Marker ELISA for the Detection of Goose T Cell Surface CD8α Molecules.

Gene.. 2016-10;

Zhao Q, Liu F, Chen S, Yan X, Qi Y, Wang M, Jia R, Zhu D, Liu M, Chen X, Cheng A.

Products/Services Used	Details	Operation
Catalog Antibody	... Purified exc-goCD8α-his protein was coated, and the non-immunogenic serum was used as negative control. All collected antiserums were purified by a protein A antibody purification kit (GenScript) according the manufacturer's protocol. ...	Get A Quote

摘要

CD8 molecule is a cell membrane glycoprotein, which plays an important role in cell-mediated immunity. Here, we identified Chinese goose CD8α (goCD8α) gene for the first time. The full-length cDNA of goCD8α is 1459bp in length and contains a 711bp open reading frame. Phylogenetic analysis shows that the waterfowl CD8α formed a monophyletic group. Semi-quantitative RT-PCR analysis showed that transcripts of goCD8α mRNA were high in the immune-related organs and mucosal immune system in gosling, and high in thymus and spleen comparing to other immune-related tissues in goose. The obvious increase of CD8α expression was observed in spleen of acute new type gosling viral enteritis virus (NGVEV) infected bird,... More

关键词

AAP; AP; AUAP; Adapter Primer; BF; BLAST; Basic Local Alignment Search Tool; CD8α; CHCD8α; CIS; CMC; CMI; CT; CTLs; GSP; Goose; HG; IEL; Immunobiological activity; LD(50); LPS; MHC; MIS; MNCs; Molecular cloning; NGVEV; NGVEV infection; OFR; PHA; PI; RACE; RT-PCR; T cell antigen receptor; TCID50; TCR; Tissue distribution; TuCD8α; UTR; abridged anchor primer; abridged universal amplification primer; bursa of fabricius; cDNA; cecal tonsil; cell-mediated cytotoxicity; cell-mediated immune; chicken CD8α; complementary to RNA; cutaneous immune system; cytotoxic T lymphocytes; gene specific primer; goCD8α; goose CD8α; harderian gland; intestinal intraepithelial lymphocytes; lethal dose; lipopolysaccharide; major histocompatibility complex; monocolor cells; mucosal immune system; new type gosling viral enteritis virus; open reading frame; phytohemagglutinin; post infection; qPCR; quantitative PCR; rapid amplification of cDNA ends; reverse transcription-polymerase chain reaction; tissue culture infective dose; turkey CD8α; untranslated region

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