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Proteins, Expression, Isolation and Analysis | ... 2.2. Protein Expression. While initial experiments were conducted with commercial CXCL8 (Genscript), follow up studies were performed with chemokine recombinantly expressed in our lab as described previously [26] and [27]. 2.3. Preparation of homogenously coated slides. ... | Get A Quote |
The attachment of neutrophils to the endothelial surface and their migration towards the site of inflammation following chemokine gradients play an essential role in the innate immune response. Chemokines adhere to glycosaminoglycans on the endothelial surface to be detected by leukocytes and trigger their movement along surface- bound gradients in a process called haptotaxis. In assays to systematically study the response of leukocytes to surface-bound compounds both the spatial arrangement of the compound as well as the mode of immobilization need to be controlled. In this study microcontact printing was employed to create patterns of hydrophobic or functionalized thiols on gold-coated glass slides and CXCL8 ... More