genscript #L00209)....">
至今,GenScript的服务及产品已被Cell, Nature, Science, PNAS等1300多家生物医药类杂志引用近万次,处于行业领先水平。NIH、哈佛、耶鲁、斯坦福、普林斯顿、杜克大学等约400家全球著名机构使用GenScript的基因合成、多肽服务、抗体服务和蛋白服务等成功地发表科研成果,再次证明GenScript 有能力帮助业内科学家Make research easy.

Enzymatic characterization of recombinant nitrate reductase expressed and purified from Neurospora crassa.

Fungal Genet Biol.. 2015-04;  80:10-18
Ringel P, Probst C, Dammeyer T, Buchmeier S, Jänsch L, Wissing J, Tinnefeld P, Mendel RR, Jockusch BM, Kruse T. Department of Plant Biology, Braunschweig University of Technology, 38106 Braunschweig, Germany.
Products/Services Used Details Operation

摘要

We established an expression and purification procedure for recombinant protein production in Neurospora crassa (N. crassa). This Strep-tag® based system was successfully used for purifying recombinant N. crassa nitrate reductase (NR), whose enzymatic activity was compared to recombinant N. crassa NR purified from Escherichia coli. The purity of the two different NR preparations was similar but NR purified from N. crassa showed a significantly higher nitrate turnover rate. Two phosphorylation sites were identified for NR purified from the endogenous expression system. We conclude that homologous expression of N. crassa NR yields a higher active enzyme and propose that NR phosphorylation causes enhanced enzy... More

关键词

Neurospora crassa; Nitrate reductase; Recombinant protein expression; Strep-tag®; Post-translational modification