GenScript (New Jersey, USA). Standard aptamer solutions were prepared by dissolving the DNA in binding buffer...">
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Label-free voltammetric aptasensor for the sensitive detection of microcystin-LR using graphene-modified electrodes.

Anal Chem.. 2014-08;  86(15):7551-7
SHH Eissa,?A Ng, M Siaj,?MM Zourob. Center of Biomedical Engineering, Cranfield University, Cranfield Campus, Bedfordshire MK43 0AL, U.K.
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摘要

The development of successful biosensing platforms is highly dependent upon the biorecognition properties of the recognition receptor and the sensitivity of the transducer of the binding signal. The integration of the high affinity and specificity of DNA aptamers with the unique properties of the carbon nanomaterial graphene offers an excellent avenue for sensitive and selective biosensing architectures. In this work, a highly sensitive and selective aptasensor which utilizes an unlabeled DNA aptamer assembled on a graphene electrode for microcystin-LR detection was developed. A facile strategy was used for the aptasensor fabrication on the basis of the noncovalent assembly of DNA aptamer on graphene-modified s... More

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