GenScript, #L00206) were used for binding proteins and washed with HEPES buffer containing 0.1% TrionX100 and 0.1 mM...">
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Identification of SUMO Substrates with Diverse Functions Using the Xenopus Egg Extract System.

Mol Cell Proteomics.. 2014-05; 
Ma L, Aslanian A, Sun H, Jin M, Shi Y, Yates JR 3rd, Hunter T. Salk Institute for Biological Studies, United States.
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摘要

Post-translational modification by SUMO is a highly conserved pathway in eukaryotes that plays very important regulatory roles in many cellular processes. Deregulation of the SUMO pathway contributes to the development and progression of many diseases including cancer. Therefore, identifying additional SUMO substrates and studying how their cellular and biological functions are regulated by sumoylation should provide new insights. Our studies showed that sumoylation activity was significant in Xenopus egg extracts, and that a high level of sumoylation was associated with sperm chromatin when SUMO was incubated with Xenopus egg extracts. By isolating SUMO-conjugated substrates using His-tagged SUMO1 or SUMO2 pro... More

关键词

Cell cycle*; Chromatin function or biology; Mass Spectrometry; Post-translational modifications*; Protein Identification*; SUMO; Signal Transduction*; Xenopus egg extract; mitosis; sumoylation