目录产品 » GenCRISPR™/Cas9 基因编辑相关产品 » GenCRISPR™ Cas9 Enzymes » GenCrispr NLS-Cas9-D10A Nickase
GenCrispr NLS-Cas9-D10A Nickase

In vitro DNA cleavage assay with GenCrispr NLS-Cas9-D10A Nickase
Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1% agarose gel. Input DNA is pUC57 plasmid DNA (OC, open circular; LIN, linearized; SC, supercoiled)

GenCrispr NLS-Cas9-D10A Nickase

GenCrispr NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 nuclease cleaves the double strand DNA generating two break sites based on its two active domains. NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease which makes one active domain deactivated, thus it can only cut one single strand DNA that is complementary to the guide-RNA, producing one single strand cut. Combined with two different gRNA, NLS-Cas9-D10A Nickase produces two cut sites respectively and causes a double strand break. Compared with the wild type Cas9 nuclease, the two-gRNA guided cleavage can significantly reduce the off target effects. Product Source: GenCripsr NLS-Cas9-D10A Nickase is highly purified mutant proteins expressed in an E. coli strain carrying a plasmid encoding the mutated Cas9 gene from Streptococcus pyogenes. Key features: DNA-free: no external DNA added to system. High cleavage efficiency: NLS ensures the efficient entry of Cas9 protein into nuclei. Low off target: Double gRNA-guided cleavage and transient expression of Cas9 nuclease. Time-saving: no need for transcription and translation. With this Cas9 Nuclease, you can: Screening the highly efficient and specific targeting gRNAs using in vitro DNA cleavage. In vivo gene editing combined with specific gRNA by electroporation or injection.
Z03390
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Description

GenCrispr NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease. Cas9 nuclease is an RNA-guided endonuclease that can catalyze cleavage of double stranded DNA. This kind of targeted nuclease is a powerful tool for genome editing with high precision. Cas9 nuclease cleaves the double strand DNA generating two break sites based on its two active domains. NLS-Cas9-D10A Nickase is a mutation form of Cas9 Nuclease which makes one active domain deactivated, thus it can only cut one single strand DNA that is complementary to the guide-RNA, producing one single strand cut. Combined with two different gRNA, NLS-Cas9-D10A Nickase produces two cut sites respectively and causes a double strand break. Compared with the wild type Cas9 nuclease, the two-gRNA guided cleavage can significantly reduce the off target effects.

Product Source: GenCripsr NLS-Cas9-D10A Nickase is highly purified mutant proteins expressed in an E. coli strain carrying a plasmid encoding the mutated Cas9 gene from Streptococcus pyogenes.

Key features:

  • DNA-free: no external DNA added to system.
  • High cleavage efficiency: NLS ensures the efficient entry of Cas9 protein into nuclei.
  • Low off target: Double gRNA-guided cleavage and transient expression of Cas9 nuclease.
  • Time-saving: no need for transcription and translation.

With this Cas9 Nuclease, you can:

  1. Screening the highly efficient and specific targeting gRNAs using in vitro DNA cleavage.
  2. In vivo gene editing combined with specific gRNA by electroporation or injection.

Note

1000 nM is equal to 160 ng/ul.

Storage & Stability GenCrispr NLS-Cas9-D10A Nickase is supplied with 1X storage buffer (10 mM Tris, 300 mM NaCl,
0.1 mM EDTA, 1 mM DTT, 50% Glycerol pH7.4 at 25°C). The recommended storage
temperature is -20°C.


  • GenCrispr NLS-Cas9-D10A Nickase
  • GenCrispr NLS-Cas9-D10A Nickase

    In vitro DNA cleavage assay with GenCrispr NLS-Cas9-D10A Nickase
    Reactions were set up according to recommended conditions, and cleavage products were resolved on a 1% agarose gel. Input DNA is pUC57 plasmid DNA (OC, open circular; LIN, linearized; SC, supercoiled)


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