The Enceed™ T cell Activation reagent induces T cell activation and expansion
in enriched T cells or PBMCs. The Enceed™ T cell Activation reagent is composed of biodegradable
matrix-coated nanoparticles conjugated with mouse anti-human CD3 and mouse
anti-human CD28 antibodies. The small sized particulate structure of the
activation reagent enables its easy removal by media washing or
centrifugation. The
recommended titer of 1:200 has been found to achieve efficient stimulation of
majority of T cell subsets. For special applications, the optimal stimulation
titer should be determined experimentally. Excessive amount of the Enceed™ T cell Activation reagent may result in
over-activation of T cells risking activation-induced cell death. During cell
expansion using the Enceed™ T
cell Activation reagent, the cells take up to 13 days after reagent application
to show optimal expansion. Restimulation after 13 days can be performed for
longer cultivation. The Enceed™ T
cell Activation reagent can be used together with cytokines such as human IL-2,
IL-15, and IL-7 for more efficient activation. |