T cells were isolated from human PBMCs using CytoSinct™ Human CD3 Nanobeads and CytoSinctTM gM columns. CD3+ cells were stained with anti- CD3 (UCHT1) PE and gated on Live/hCD45+.
CytoSinct™ CD3 Nanobeads, human
L00896 | |
|
|
¥1875 | |
|
|
|
|
联系我们 |
T cells were isolated from human PBMCs using CytoSinct™ Human CD3 Nanobeads and CytoSinctTM gM columns. CD3+ cells were stained with anti- CD3 (UCHT1) PE and gated on Live/hCD45+.
L00896 | |
|
|
¥1875 | |
|
|
|
|
联系我们 |
The CytoSinct™ CD3 Nanobeads, human are used for
separating CD3+ cells from fresh or frozen peripheral blood mononuclear cells
(PBMCs), leukapheresis products or single cell suspension based on the surface
expression of human CD3. The surface of Nanobeads are labeled with anti-human
CD3 monoclonal antibody. To begin the isolation, Nanobeads are added to the
cells. The CD3 antibodies preconjugated to the Nanobeads can bind the target
cells expressing CD3 on cell surface. The cells/beads mixed suspension is
loaded onto a CytoSinctTM Column which is
effectively magnetized by an external magnetic field from CytoSinct™ Magnet, or other
compatible cell isolation columns and magnets. The Nanobeads-labeled CD3+ cells
are retained within the column and enriched during the wash step when Isolation
Buffer is used to flush out the CD3- cells. After removing magnetic field, the
target CD3+ cells can be easily eluted from the column. |
For research use only. Not intended for human and animal therapeutic or diagnostic use.
Reactivity |
Human |
Formulation |
Bio-degradable matrix coated nanoparticle conjugated with anti-CD3
antibodies supplied in phosphate buffered-saline (PBS), containing Human Serum
Albumin (HSA), pH 7.0-7.4. |
Storage |
Store at 2-8 °C. Do not freeze. |
Positive selection or depletion of CD3+ T cells
from leukapheresis, PBMC or cell cultures. Isolated CD3+ T cells can be used
for culture and expansion, flow cytometry, T cells functional assays. |