目录产品 » Anti-HA IP Resin
Anti-HA IP Resin

Lanes:
M.Protein Marker
1. Whole cell lysate
2. HA tagged fusion protein was precipitated by Anti-HA IP Resin

Anti-HA IP Resin

Anti-HA IP Resin, with high specificity and affinity to HA tag, is ideal for quick and efficient immunoprecipitation of HA-tagged protein or HA-tagged protein-target protein complex.
L00777
¥4000

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Description

Principle
Add the sample containing the HA-tagged protein to the Anti-HA IP Resin and allow the protein bind to the Resin during a short time incubation. Then the HA-tagged protein can be eluted off the beads, or used directly for pull-down of target protein.


Material Supplied
Genscript Anti-HA IP Resin is an agarose beads coupled to THE™ HA Tag Antibody, mAb, Mouse (Genscript, A01244). The antibody recognizes C-terminal, N-terminal, and internal HA tagged-fusion proteins. It is supplied as a suspension in 50% glycerol containing 10 mM sodium phosphate, 150 mM sodium chloride, pH 7.4, 0.02 % (w/v) sodium azide (PBS/A).
Storage and Stability
Store the resin: Anti-HA IP Resin is supplied at -20°C.Do not store the gel at freezing temperatures in the absence of glycerol. 
Store the Column: Wash the column three times with 5 ml of TBS/A (TBS containing 0.02 % sodium azide) then add another 5 ml of TBS/A and store at 2-8°C without draining.
Additional Buffers Needed
Equilibration buffer:  50 mM Tris, 150 mM NaCl, pH 7.4.
1×SDS Sample Buffer: 62.5 mM Tris-HCl (pH 6.8 at 25°C), 2% w/v SDS, 10% glycerol, 50 mM DTT, 0.01% w/v bromophenol blue.

Product Information Characteristics of Anti-HA IP Resin
Resin Volume1 ml settled resin (2 ml 50% slurry)
LigandAnti-HA monoclonal antibody against HA tag
Number of HA-tagged protein per ligand2
Binding capacityApproximately 1 mg HA-tagged protein (~20kDa) per ml settled resin
Storage solution1X PBS containing 50 % glycerol
Storage and stabilityStore at -20 °C for up to 12 months.
Resin reuseThe resin can be recycled for at least 3 times. If maintained properly, the resin can be reused 10 times with minimum loss of binding capability.

  • Anti-HA IP Resin
  • Anti-HA IP Resin

    Lanes:
    M.Protein Marker
    1. Whole cell lysate
    2. HA tagged fusion protein was precipitated by Anti-HA IP Resin



For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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